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Yélamos, Belén ; Núñez, Elena ; Gómez-Gutiérrez, Julián ; Datta, Manisha ; Pacheco, Beatriz ; Peterson, Darrell L. ; Gavilanes, Francisco | Wiley-Blackwell | 1999-12The gene coding for the major capsid protein of feline immunodeficiency virus (FIV) has been cloned into the expression vector pQE60, which allows protein purification by affinity chromatography on a nitrilotriacetic acid/Ni/agarose column. The [...]texto impreso
Tello, Daniel ; Rodríguez-Rodríguez, Mar ; Yélamos, Belén ; Gómez-Gutiérrez, Julián ; Ortega, Sara ; Pacheco, Beatriz ; Peterson, Darrell L. ; Gavilanes, Francisco | Academic Press Inc. Elsevier Science | 2010-06Hepatitis C virus encodes two enveloped glycoproteins, E1 and E2, which are involved in viral attachment and entry into target cells. We have obtained in insect cells infected by recombinant baculovirus a chimeric secreted recombinant protein, E[...]texto impreso
Tello, Daniel ; Rodríguez-Rodríguez, Mar ; Ortega, Sara ; Lombana, Laura ; Yélamos, Belén ; Gómez-Gutiérrez, Julián ; Peterson, Darrell L. ; Gavilanes, Francisco | Wiley-Blackwell | 2014-06-01We have used an isolated chimeric protein E1340E2661 that includes the ectodomains of the envelope proteins of epatitis C virus to study its interaction with model membranes. E1340E2661 has some of the membrane destabilization properties, vesicl[...]texto impreso
Tello, Daniel ; Rodríguez-Rodríguez, Mar ; Yélamos, Belén ; Gómez-Gutiérrez, Julián ; Peterson, Darrell L. ; Gavilanes, Francisco | Elsevier Science BV | 2015-03-01In this report it is described for the first time the expression and purification of large quantities of a oluble and correctly folded chimeric recombinant protein, E2661E1340, containing the permuted Hepatitis C virus (HCV) glycoprotein ectodom[...]texto impreso
Yélamos, Belén ; Roncal, Fernando ; Albar, Juan P. ; Rodríguez-Crespo, Ignacio ; Gavilanes, Francisco | American Chemical Society | 2006-01-06Endothelial Nitric Oxide Synthase (eNOS) is unique among the NOS isoforms in its being dually acylated by the fatty acids myristate and palmitate. Due to its N-erminus consensus sequence, eNOS becomes cotranslationally myristoylated through an a[...]texto impreso
Núñez, Elena ; Yélamos, Belén ; Delgado, Carmen ; Gómez-Gutiérrez, Julián ; Peterson, Darrell L. ; Gavilanes, Francisco | Elsevier Science BV | 2009-02The role of preS domains of the hepatitis B virus (HBV) envelope proteins in the first steps of viral infection has been restricted to their implication in virus attachment to a putative hepatocyte receptor. In order to explore a fusion activity[...]texto impreso
Pacheco, Beatriz ; Gómez-Gutiérrez, Julián ; Yélamos, Belén ; Delgado, Carmen ; Roncal, Fernando ; Albar, Juan P. ; Peterson, Darrell ; Gavilanes, Francisco | Elsevier Science BV | 2006-06Based on the predicted capacity to interact with membranes at the interface, we have found three regions in the ectodomain of the hepatitis C virus envelope glycoprotein E2 (430-449, 543-560 and 603-624) with the ability to destabilize membranes[...]texto impreso
Ortega-Atienza, Sara ; Lombana, Laura ; Gómez-Gutiérrez, Julián ; Yélamos, Belén ; Peterson, Darrell L. ; Gavilanes, Francisco | Academic Press Inc.Elsevier Science | 2014-12Hepatitis C virus (HCV) envelope glycoproteins, E1 and E2, are involved in the first steps of virus infection. The E2 ectodomain can be produced as an isolated form (E2661). However, there is some concern about its proper conformation and the ro[...]texto impreso
Delgado, Carmen L. ; Núñez, Elena ; Yélamos, Belén ; Gómez-Gutiérrez, Julián ; Peterson, Darrell L. ; Gavilanes, Francisco | American Chemical Society | 2012-10-09In order to shed light on the hepatitis B virus fusion mechanism and to explore the fusogenic capabilities of preS regions, a recombinant duck hepatitis B virus (DHBV) preS protein (DpreS) containing six histidines at the carboxyterminal end has[...]texto impreso
Rodríguez-Rodríguez, Mar ; Tello, Daniel ; Yélamos, Belén ; GómezGutiérrez, Julián ; Pacheco, Beatriz ; Ortega, Sara ; Serrano, Alicia G. ; Peterson, Darrell L. ; Gavilanes, Francisco | Elsevier Science BV | 2009-01We describe the structural and antigenic properties of a soluble form of hepatitis C virus E2 envelope protein ectodomain ending at residue 661 (E2661) which is obtained in large quantities in a baculovirus/insect cell system. The protein is sec[...]texto impreso
Delgado, Carmen L. ; Núñez, Elena ; Yélamos, Belén ; Gómez-Gutiérrez, Julián ; Peterson, Darrell L. ; Gavilanes, Francisco | Elsevier Science BV | 2015-04In a previous study, it was shown that purified preS domains of hepatitis B virus (HBV) could interact with acidic phospholipid vesicles and induce aggregation, lipid mixing and leakage of internal contents which could be indicative of their inv[...]texto impreso
Lombana, Laura ; Ortega Atienza, Sara ; Gómez Gutierrez, Julián ; Yélamos, Belén ; Peterson, Darrell ; Gavilanes, Francisco | Elsevier | 2016-03-06We have obtained a chimeric protein containing the ectodomains of hepatitis C virus (HCV) envelope proteins but lacking the region 268-292 of E1. All its structural properties are coincident with those of the corresponding full length chimera. T[...]texto impreso
Yélamos, Belén ; Núñez, Elena ; Gómez-Gutiérrez, Julián ; Delgado, Carmen ; Pacheco, Beatriz ; Peterson, Darrell L. ; Gavilanes, Francisco | Elsevier Science BV | 2001-03-09Circular dichroism and fluorescence spectroscopy have been employed to study the urea unfolding mechanism of a recombinant form of the major core protein of feline immunodeficiency virus (FIV-rp24) and its native tryptophan mutants. The equilibr[...]
