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Yélamos, Belén ; Núñez, Elena ; Gómez-Gutiérrez, Julián ; Datta, Manisha ; Pacheco, Beatriz ; Peterson, Darrell L. ; Gavilanes, Francisco | Wiley-Blackwell | 1999-12The gene coding for the major capsid protein of feline immunodeficiency virus (FIV) has been cloned into the expression vector pQE60, which allows protein purification by affinity chromatography on a nitrilotriacetic acid/Ni/agarose column. The [...]texto impreso
Tello, Daniel ; Rodríguez-Rodríguez, Mar ; Yélamos, Belén ; Gómez-Gutiérrez, Julián ; Ortega, Sara ; Pacheco, Beatriz ; Peterson, Darrell L. ; Gavilanes, Francisco | Academic Press Inc. Elsevier Science | 2010-06Hepatitis C virus encodes two enveloped glycoproteins, E1 and E2, which are involved in viral attachment and entry into target cells. We have obtained in insect cells infected by recombinant baculovirus a chimeric secreted recombinant protein, E[...]texto impreso
Pacheco, Beatriz ; Gómez-Gutiérrez, Julián ; Yélamos, Belén ; Delgado, Carmen ; Roncal, Fernando ; Albar, Juan P. ; Peterson, Darrell ; Gavilanes, Francisco | Elsevier Science BV | 2006-06Based on the predicted capacity to interact with membranes at the interface, we have found three regions in the ectodomain of the hepatitis C virus envelope glycoprotein E2 (430-449, 543-560 and 603-624) with the ability to destabilize membranes[...]texto impreso
Rodríguez-Rodríguez, Mar ; Tello, Daniel ; Yélamos, Belén ; GómezGutiérrez, Julián ; Pacheco, Beatriz ; Ortega, Sara ; Serrano, Alicia G. ; Peterson, Darrell L. ; Gavilanes, Francisco | Elsevier Science BV | 2009-01We describe the structural and antigenic properties of a soluble form of hepatitis C virus E2 envelope protein ectodomain ending at residue 661 (E2661) which is obtained in large quantities in a baculovirus/insect cell system. The protein is sec[...]texto impreso
Yélamos, Belén ; Núñez, Elena ; Gómez-Gutiérrez, Julián ; Delgado, Carmen ; Pacheco, Beatriz ; Peterson, Darrell L. ; Gavilanes, Francisco | Elsevier Science BV | 2001-03-09Circular dichroism and fluorescence spectroscopy have been employed to study the urea unfolding mechanism of a recombinant form of the major core protein of feline immunodeficiency virus (FIV-rp24) and its native tryptophan mutants. The equilibr[...]
