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Reche, Pedro A ; Arrebola, R ; Olmo, A ; Santi, D V ; González-Pacanowska, D. ; Ruiz Pérez, Luis Miguel | Elsevier | 1994We have cloned, sequenced and expressed the Trypanosoma cruzi gene encoding the bifunctional protein dihydrofolate reductase-thymidylate synthase (DHFR-TS). The strategy followed for the isolation of positive clones from a genomic library was ba[...]texto impreso
Reche, Pedro A ; Arrebola, R ; Santi, D V ; González-Pacanowska, D. ; Ruiz Pérez, Luis Miguel | Elsevier | 1995We have cloned and expressed in Escherichia coli a 702-base pair gene coding for the dihydrofolate reductase (DHFR) domain of the bifunctional dihydrofolate reductase-thymidylate synthase (DHFR-TS) from Trypanosoma cruzi. The DHFR domain was pur[...]texto impreso
Arrebola, R ; Olmo, A ; Reche, Pedro A ; Garvey, Edward P. ; Santi, D V ; Ruiz Pérez, Luis Miguel ; González-Pacanowska, D. | American Society for Biochemistry and Molecular Biology | 1994-04-08The MTX-resistant Leishmania major promastigote cell line D7BR1000 displays extrachromosomal amplified R-region DNA, which contains the gene for dihydrofolate reductase-thymidylate synthase (DHFR-TS) (Garvey, E. P., and Santi, D. V. (1986) Scien[...]texto impreso
Arrebola, R ; Olmo, A ; Reche, Pedro A ; Garvey, Edward P. ; Santi, D V ; Ruiz Pérez, Luis Miguel ; González-Pacanowska, D. | 1994The MTX-resistant Leishmania major promastigote cell line D7BR1000 displays extrachromosomal amplified R-region DNA, which contains the gene for dihydrofolate reductase-thymidylate synthase (DHFR-TS) (Garvey, E. P., and Santi, D. V. (1986) Scien[...]texto impreso
Moreno-Murcia, Juan Antonio ; Martínez-Galindo, Mª Celestina ; Ruiz Pérez, Luis Miguel ; García Coll, Virginia ; Martín-Albo, José | Editorial Universidad de Almería | 2017-04-27Abstract: The main purpose of the present study was to evaluate the validity of the Spanish version of the Children’s Self-Perceptions of Adequacy in and Predilection for Physical Activity questionnaire (Hay, 1992). Two studies were carried out [...]
