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Esteban Fernández, Diego ; El-Khatib, Ahmed H. ; Moraleja San José, Irene ; Gómez Gómez, M.Milagros ; Linscheid, Michael W. | ACS | 2014-12-22Molecular mass spectrometry has been applied to simultaneously obtain molecular and elemental information from metal-containing species. Energy tuning of the higher-energy collision dissociation (HCD) fragmentation cell allows the controlled pro[...]texto impreso
Aboulmagd, Sarah ; Esteban Fernández, Diego ; Moreno Gordaliza, Estefanía ; Neumann, Boris ; El-Khatib, A.H. ; Lázaro, Alberto ; Tejedor, Alberto ; Gómez Gómez, M.Milagros ; Linscheid, Michael W. | ACS | 2017The quest for internal standards useful in MALDI imaging studies goes on to get not only lateral distribution but also reliable relative quantitative information. We developed a method based on application of matrix and dual internal standards t[...]texto impreso
Moreno Gordaliza, Estefanía ; Esteban Fernández, Diego ; Giesen, Charlotte ; Lehmann, Karola ; Lázaro, Alberto ; Tejedor, Alberto ; Scheler, Christian ; Cañas Montalvo, Benito ; Jakubowski, Norbert ; Linscheid, Michael W. ; Gómez Gómez, M.Milagros | RSC | 2012A method for the analysis of Pt–protein complexes in biological samples, previously subjected to cisplatin treatment, has been developed. Proteins were separated by gel electrophoresis, and those bound to Pt were detected with high sensitivity b[...]texto impreso
Moreno Gordaliza, Estefanía ; Esteban Fernández, Diego ; Lázaro, Alberto ; Aboulmagd, Sarah ; Humanes, Blanca ; Linscheid, Michael W. ; Gómez Gómez, M.Milagros | American Society for Biochemistry and Molecular Biology Inc. | 2018-07Nephrotoxicity is a major limitation to cisplatin antitumor therapies. Cilastatin, an inhibitor of renal dehydropeptidase-I, was recently proposed as a promising nephroprotector against cisplatin toxicity, preventing apoptotic cell death. In thi[...]texto impreso
Moreno Gordaliza, Estefanía ; Esteban Fernández, Diego ; Lázaro, Alberto ; Humanes, Blanca ; Aboulmagd, Sarah ; Tejedor, Alberto ; Linscheid, Michael W. ; Gómez Gómez, M.Milagros | Elsevier | 2017Imaging techniques for mapping molecular distributions in tissue sections can reveal valuable information on biomolecules involved in relevant biochemical processes. A method has been developed for comprehensive, reproducible and sensitive lipid[...]
