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Castellanos, Elena ; Aranaz Martín, Alicia ; Gould, Katherine A ; Linedale, Richard ; Stevenson, Karen ; Álvarez Sánchez, Julio ; Domínguez Rodríguez, Lucas ; Juan Ferré, Lucia de ; Hinds, Jason ; Bull, Tim J | American Society for Microbiology | 2009-02Mycobacterium avium subsp. paratuberculosis is an important animal pathogen widely disseminated in the environment that has also been associated with Crohn's disease in humans. Three M. avium subsp. paratuberculosis genomotypes are recognized, b[...]texto impreso
Castellanos, Elena ; Aranaz Martín, Alicia ; De Buck, Jeroen | American Society for Microbiology | 2010-04High-resolution melt (HRM) analysis can identify sequence polymorphisms by comparing the melting curves of amplicons generated by real-time PCR amplification. We describe the application of this technique to identify Mycobacterium avium subspeci[...]texto impreso
Castellanos, Elena ; Aranaz Martín, Alicia ; Juan Ferré, Lucia de ; Álvarez Sánchez, Julio ; Rodriguez, Sabrina ; Romero Martínez, Beatriz ; Bezos Garrido, Javier ; Stevenson, Karen ; Mateos García, Ana ; Domínguez Rodríguez, Lucas | American Society for Microbiology | 2009-07Insertion sequence IS900 is used as a target for the identification of Mycobacterium avium subsp. paratuberculosis. Previous reports have revealed single nucleotide polymorphisms within IS900. This study, which analyzed the IS900 sequences of a [...]
