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Rapid identification and differentiation of Mycobacterium avium subspecies paratuberculosis types by use of real-time PCR and high-resolution melt analysis of the MAP1506 locus

Castellanos, Elena ; Aranaz Martín, Alicia ; De Buck, Jeroen | American Society for Microbiology | 2010-04

High-resolution melt (HRM) analysis can identify sequence polymorphisms by comparing the melting curves of amplicons generated by real-time PCR amplification. We describe the application of this technique to identify Mycobacterium avium subspeci[...]

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